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dc.contributor.authorVerjee, Sheela
dc.contributor.authorKelber, Olaf
dc.contributor.authorKolb, Christiane
dc.contributor.authorAbdel-Aziz, Heba
dc.contributor.authorButterweck, Veronika
dc.date.accessioned2018-01-09T12:42:03Z
dc.date.available2018-01-09T12:42:03Z
dc.date.issued2017
dc.identifier.doi10.1111/jphp.12717
dc.identifier.urihttp://hdl.handle.net/11654/25765
dc.description.abstractObjectives The major aim of this study was to get a detailed understanding of the exposure and fate of hypericin in the Caco-2 cell system when combined with various flavonoids, mixtures of flavonoids or Hypericum perforatum extract matrix (STW3-VI). Methods The permeation characteristics of hypericin in the absence or presence of quercetin, quercitrin, isoquercitrin, hyperoside and rutin were tested. Hypericin (5 μm) was mixed with single flavonoids (20 μm) or with different flavonoid combinations (each flavonoid 4 or 10 μm, total flavonoid concentration: 20 μm). Further, the uptake of hypericin (5 μm) in the presence of H. perforatum extract matrix (7.25, 29 and 58 μg/ml) was studied. Key findings Following application of hypericin to the apical side of the monolayer, only negligible amounts of the compound were found in the basolateral compartment. From all tested flavonoids, only quercitrin increased the basolateral amount of hypericin. Dual flavonoid combinations were not superior compared to the single combinations. The amount of hypericin in the basolateral compartment increased concentration-dependently in the presence of extract matrix (from 0 to 7.5%). Conclusion Comparing the effects of various flavonoid mixtures vs the extract matrix, it can be concluded that, besides flavonoids, the extract seems to contain further compounds (e.g. phenolic acids or proanthocyanidins) which substantially improve the permeation characteristics of hypericin.
dc.description.urihttp://onlinelibrary.wiley.com/doi/10.1111/jphp.12717/epdf
dc.language.isoen_US
dc.relation.ispartofJournal of Pharmacy and Pharmacology
dc.accessRightsAnonymous
dc.subjectabsorption
dc.subjectCaco-2 ce lls
dc.subjectflavonoids
dc.subjecthypericin
dc.subjectHypericum perforatum
dc.titlePermeation characteristics of hypericin across Caco-2 monolayers in presence of singe flavonoids, defined flavonoid mixtures or Hypericum extract matrix
dc.type01 - Zeitschriftenartikel, Journalartikel oder Magazin
dc.audienceScience
fhnw.publicationStatePublished
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.InventedHereYes
fhnw.PublishedSwitzerlandYes
fhnw.IsStudentsWorkno
fhnw.publicationOnlineJa


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