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dc.contributor.authorKropf, C.
dc.contributor.authorSegner, H.
dc.contributor.authorFent, K.
dc.date.accessioned2017-01-23T05:42:57Z
dc.date.available2017-01-23T05:42:57Z
dc.date.issued2016-03-02
dc.identifier.doi10.1016/j.cbpc
dc.identifier.urihttp://hdl.handle.net/11654/24033
dc.description.abstractEmbryos of oviparous fish, in contrast to (ovo) viviparous species, develop in the aquatic environment, and therefore need solute transport systems at their body surfaces for maintaining internal homeostasis and defending against potentially harmful substances. We hypothesized that solute transporters undergo changes in tissue distribution from the embryo to the larval stage. We therefore studied the mRNA profiles of eight ABC transporters (abcb1a, abcb1b, abcc1, abcc2, abcc3, abcc4, abcc5, abcg2) and three solute carriers (oatp1d, putative oatp2 putative, mate1) in different body regions (head, yolk sac epithelium, abdominal viscera, skin/muscles) of developing rainbow trout. Additionally, we investigated mRNA levels of phase I (cyp1a, cyp3a) and phase II (gstp, putative ugt1, putative ugt2) biotransformation enzymes. The study covered the developmental period from the eleuthero-embryo stage to the first-feeding larval stage (1-20days post-hatch, dph). At 1dph, transcripts of abcc2, abcc4, abcg2, cyp3a, gstp, putative mate1, and putative oatp2 occurred primarily in the yolk sac epithelium, whereas at later stages expression of these genes was predominantly observed in the abdominal viscera. The functional activity of ABC transporters in fish early life stages was assessed by rhodamine B accumulation assays. Finally, we investigated the potential impact of xenobiotics (clotrimazole, clofibric acid) on the ABC and biotransformation systems of trout early life stages. While clofibric acid had no effect, clotrimazole lead to an increased rhodamine B accumulation. The results provide evidence that the transition from the eleuthero-embryo to the larval stage is accompanied by a major alteration in tissue expression of ABC transporters.
dc.language.isoen
dc.relation.ispartofComparative Biochemistry and Physiology, Part C
dc.accessRightsAnonymous
dc.subjectYolk sac
dc.subjectSolute carriers
dc.subjectOncorhynchus mykiss
dc.subjectEarly life stage
dc.subjectBiotransformation
dc.subjectABC transporter
dc.titleABC transporters and xenobiotic defense systems in early life stages of rainbow trout (Oncorhynchus mykiss)
dc.type01 - Zeitschriftenartikel, Journalartikel oder Magazin
dc.volume185-186
dc.audienceScience
fhnw.publicationStatePublished
fhnw.ReviewTypeAnonymous ex ante peer review of a complete publication
fhnw.InventedHereYes
fhnw.PublishedSwitzerlandNo
fhnw.pagination45-56
fhnw.IsStudentsWorkno
fhnw.publicationOnlineJa


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